Phylogenetic relationships of Shiga toxin-producing Escherichia coli isolated from Peruvian children

  1. Contreras, C.A. 7
  2. Ochoa, T.J. 711
  3. Ruiz, J. 23
  4. Lacher, D.W. 12
  5. Rivera, Fulton P. 7
  6. Saenz, Y. 1
  7. Chea-Woo, E. 7
  8. Zavaleta, Nelly . 6
  9. Gil, A.I. 6
  10. Lanata, C.F. 56
  11. Huicho, L. 7810
  12. Maves, R.C. 13
  13. Torres, C. 19
  14. DebRoy, C. 4
  15. Cleary, T.G. 11
  1. 1 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  2. 2 Institut d'Investigacions Biomèdiques August Pi i Sunyer
    info

    Institut d'Investigacions Biomèdiques August Pi i Sunyer

    Barcelona, España

    ROR https://ror.org/054vayn55

  3. 3 Centro de Investigación Biomédica en Red sobre Epidemiología Y Salud Pública
    info

    Centro de Investigación Biomédica en Red sobre Epidemiología Y Salud Pública

    Madrid, España

    ROR https://ror.org/050q0kv47

  4. 4 Pennsylvania State University
    info

    Pennsylvania State University

    State College, Estados Unidos

    ROR https://ror.org/04p491231

  5. 5 Universidad Peruana de Ciencias Aplicadas
    info

    Universidad Peruana de Ciencias Aplicadas

    Lima, Perú

    ROR https://ror.org/047xrr705

  6. 6 Instituto de Investigación Nutricional, Lima, Peru
  7. 7 Universidad Peruana Cayetano Heredia
    info

    Universidad Peruana Cayetano Heredia

    Lima, Perú

    ROR https://ror.org/03yczjf25

  8. 8 Instituto Nacional de Salud del Niñ o, Lima, Peru
  9. 9 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  10. 10 Universidad Nacional Mayor de San Marcos
    info

    Universidad Nacional Mayor de San Marcos

    Lima, Perú

    ROR https://ror.org/006vs7897

  11. 11 University of Texas School of Public Health, Houston, United States
  12. 12 US Food and Drug Administration, Laurel, Maryland, United States
  13. 13 US Naval Medical Research Center Detachment, Department of Bacteriology, Lima, Peru
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Revista:
Journal of Medical Microbiology

ISSN: 0022-2615

Año de publicación: 2011

Volumen: 60

Número: 5

Páginas: 639-646

Tipo: Artículo

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DOI: 10.1099/JMM.0.026666-0 PMID: 21292859 SCOPUS: 2-s2.0-79955657794 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Medical Microbiology

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Resumen

The aim of this study was to determine the prevalence, virulence factors (stx, eae, ehxA and astA) and phylogenetic relationships [PFGE and multilocus sequence typing (MLST)] of Shiga toxinproducing Escherichia coli (STEC) strains isolated from four previous cohort studies in 2212 Peruvian children aged,36 months. STEC prevalence was 0.4% (14/3219) in diarrhoeal and 0.6% (15/2695) in control samples. None of the infected children developed haemolytic uraemic syndrome (HUS) or other complications of STEC. stx1 was present in 83% of strains, stx2 in 17%, eae in 72%, ehxA in 59% and astA in 14%. The most common serotype was O26: H11 (14%) and the most common seropathotype was B (45%). The strains belonged mainly to phylogenetic group B1 (52%). The distinct combinations of alleles across the seven MLST loci were used to define 13 sequence types among 19 STEC strains. PFGE typing of 20 STEC strains resulted in 19 pulsed-field patterns. Comparison of the patterns revealed 11 clusters (I-XI), each usually including strains belonging to different serotypes; one exception was cluster VI, which gathered exclusively seven strains of seropathotype B, clonal group enterohaemorrhagic E. coli (EHEC) 2 and phylogenetic group B1. In summary, STEC prevalence was low in Peruvian children with diarrhea in the community setting. The strains were phylogenetically diverse and associated with mild infections. However, additional studies are needed in children with bloody diarrhoea and HUS.