Identification of novel vga(A)-carrying plasmids and a Tn5406-like transposon in meticillin-resistant Staphylococcus aureus and Staphylococcus epidermidis of human and animal origin

  1. Lozano, C. 1
  2. Aspiroz, C. 3
  3. Rezusta, A. 4
  4. Gómez-Sanz, E. 1
  5. Simon, C. 2
  6. Gómez, P. 1
  7. Ortega, C. 2
  8. Revillo, M.J. 4
  9. Zarazaga, M. 1
  10. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidad de Zaragoza
    info

    Universidad de Zaragoza

    Zaragoza, España

    ROR https://ror.org/012a91z28

  3. 3 Servicio Microbiología, Hospital Royo Villanova, Zaragoza, Spain
  4. 4 Hospital Miguel Servet
    info

    Hospital Miguel Servet

    Zaragoza, España

    ROR https://ror.org/01r13mt55

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Revista:
International Journal of Antimicrobial Agents

ISSN: 0924-8579

Año de publicación: 2012

Volumen: 40

Número: 4

Páginas: 306-312

Tipo: Artículo

DOI: 10.1016/J.IJANTIMICAG.2012.06.009 SCOPUS: 2-s2.0-84866043274 WoS: WOS:000308707500004 GOOGLE SCHOLAR

Otras publicaciones en: International Journal of Antimicrobial Agents

Proyectos relacionados

Detección y caracterización genética de Staphylococcus aureus resistente a meticilina en distintos ecosistemas: humanos, animales, alimentos y medioambiente. Impicaciones en salud humana.

2009/00204/001

Resumen

Nine staphylococcal strains of human and animal origin with a lincomycin-resistant/erythromycin-susceptible phenotype and carrying vga genes were characterised to determine the genetic elements involved in the dissemination of these uncommon resistance genes. These strains were typed by multilocus sequence typing (MLST), staphylococcal cassette chromosome mec (SCCmec) and/or spa typing. Antimicrobial susceptibility was studied by disk diffusion and agar dilution methods. Presence of the genes lnu(A), lnu(B), vga(A), vga(A)v, vga(B), vga(C), vga(E), lsa(B) and cfr was studied by PCR. Transformation experiments were carried out in all strains, and the plasmid or chromosomal gene location was determined by Southern blot analysis. Genetic environments of the vga genes were analysed by PCR mapping or inverse PCR and sequencing. Five meticillin-resistant Staphylococcus aureus (MRSA) ST398 strains and three Staphylococcus epidermidis strains harboured the gene vga(A), and one MRSA-ST8 strain contained the gene vga(A)v. One MRSA-ST398 strain, which also contained the gene lnu(A), showed the highest minimum inhibitory concentration (MIC) to lincomycin. The vga(A)v-positive strain presented lower MIC values than the vga(A)-positive strains. Presence of the pVGA plasmid was confirmed in two MRSA-ST398 strains. Four novel vga(A)-carrying plasmids were detected: pUR2355 (in two MRSA and one meticillin-susceptible S. epidermidis); pUR4128 (one MRSA); pUR3036 [one meticillin-resistant S. epidermidis (MRSE)]; and pUR3937 (one MRSE). The plasmid pUR4128 was very similar to pUR2355. Plasmids pUR3036 and pUR3937 were related and were very similar to plasmid pSE-12228-06. The gene vga(A)v was located in a transposon analogous to Tn5406. Therefore, four novel vga(A)-carrying plasmids and a variant of Tn5406 were identified in this study. © 2012 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.