Mechanisms of antibiotic resistance in Escherichia coli isolates recovered from wild animals

  1. Costa, D. 13
  2. Poeta, P. 23
  3. Sáenz, Y. 1
  4. Vinué, L. 1
  5. Coelho, A.C. 3
  6. Matos, M. 34
  7. Rojo-Bezares, B. 1
  8. Rodrigues, J. 23
  9. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Estudos de Ciência Animal e Veterinária, Vila Real, Portugal
  3. 3 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  4. 4 Departamento de Genética e Biotecnología, Instituto de Biotecnología e Bioengenharia, Vila Real, Portugal
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Revista:
Microbial Drug Resistance

ISSN: 1076-6294

Año de publicación: 2008

Volumen: 14

Número: 1

Páginas: 71-77

Tipo: Artículo

DOI: 10.1089/MDR.2008.0795 PMID: 18321208 SCOPUS: 2-s2.0-41149129305 WoS: WOS:000254870900011 GOOGLE SCHOLAR

Otras publicaciones en: Microbial Drug Resistance

Resumen

Seventy-two fecal samples obtained from wild animals in Portugal were sampled on Levine agar plates (nonsupplemented with antibiotics), and Escherichia coli isolates were recovered from 56 of them (78%), obtaining a total of 112 E. coli isolates (two per sample). Susceptibility to 16 antibiotics was studied in these isolates, and the following percentages of resistance were obtained: tetracycline, streptomycin, ampicillin, and trimethoprim- sulfamethoxazole (SXT) (range 19-35%); nalidixic acid (14%); ciprofloxacin (9%); amoxicillin-clavulanic acid, gentamicin, tobramycin, and chloramphenicol (range 4.5-7%); cefotaxime, and aztreonam (1.8%); ceftazidime (0.9%); and amikacin, cefoxitin, and imipenem (0%). A bla TEM gene was found in 22 of the 25 ampicillin-resistant isolates, and the gene encoding CTX-M-14 β-lactamase was identified in the two cefotaxime-resistant isolates (recovered from a common kestrel and a sparrowhawk), associated with bla TEM-52 gene in one of them. Other resistance genes detected were as follows: aac(3)-II or aac(3)-IV genes in all gentamicin-resistant isolates; aadA1 or aadA2 in 22 of 25 streptomycin-resistant isolates; tet(A) and/or tet(B) in all 39 tetracycline-resistant isolates; and sul1 and/or sul2 and/or sul3 genes in all 21 SXT-resistant isolates. Two amino acid changes in GyrA protein (Ser83Leu + Asp87Asn) and one change in ParC protein (Ser80Ile) were identified in all 10 ciprofloxacin-resistant isolates of our series. The intestinal tract of wild animals is a reservoir of antibiotic resistance genes, especially for ampicillin, tetracycline, streptomycin, and SXT, and it is also remarkable that multiresistant E. coli isolates are detected in some of the tested animals. © Copyright 2008, Mary Ann Liebert, Inc.