Prevalence of antimicrobial resistance and resistance genes in faecal Escherichia coli isolates recovered from healthy pets

  1. Costa, D. 14
  2. Poeta, P. 24
  3. Sáenz, Y. 1
  4. Coelho, A.C. 4
  5. Matos, M. 34
  6. Vinué, L. 1
  7. Rodrigues, J. 24
  8. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Estudos de Ciência Animal e Veterinária, Vila Real, Portugal
  3. 3 Departamento de Genética e Biotecnología, Instituto de Biotecnología e Bioengenharia, Vila Real, Portugal
  4. 4 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

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Revista:
Veterinary Microbiology

ISSN: 0378-1135

Año de publicación: 2008

Volumen: 127

Número: 1-2

Páginas: 97-105

Tipo: Artículo

DOI: 10.1016/J.VETMIC.2007.08.004 PMID: 17870255 SCOPUS: 2-s2.0-37249078006 WoS: WOS:000252578200011 GOOGLE SCHOLAR

Otras publicaciones en: Veterinary Microbiology

Resumen

Faecal samples of healthy dogs (n = 39) and cats (n = 36) obtained in Northern Portugal were seeded on Levine agar plates, and two Escherichia coli isolates per sample were recovered (78 of dogs and 66 of cats). The susceptibility to 16 antimicrobial agents was tested in this series of 144 E. coli isolates. Almost 20% of them showed tetracycline resistance and 12 and 15% presented ampicillin or streptomycin resistance, respectively. The percentage of resistance to the other antimicrobial agents was in all cases below 4%, and no resistant isolates were detected for ceftazidime, imipenem, cefoxitin or amikacin. Two isolates (from one dog) showed cefotaxime-resistance and harboured both the CTX-M-1 and OXA-30 β-lactamases. A bla TEM gene was detected in 12 of 17 ampicillin-resistant isolates, the aac(3)-II gene in the three gentamicin-resistant isolates, aadA in 7 of 22 streptomycin-resistant isolates, and tet(A) and/or tet(B) gene in all 28 tetracycline-resistant isolates. The gene encoding class 1 integrase was detected in six E. coli isolates, including the four trimethoprim-sulfamethoxazole-resistant isolates and those two harbouring CTX-M-1 and OXA-30 β-lactamases; different gene cassette arrangements were identified: dfrA1 + aadA1 (two isolates), dfrA12 + orfF + aadA2 (two isolates) and bla OXA30 + aadA1 (two isolates). One amino acid change in GyrA protein (Ser83Leu or Asp87Tyr) was detected in four nalidixic acid-resistant and ciprofloxacin-susceptible isolates and two amino acid changes in GyrA (Ser83Leu + Asp87Asn) and one in ParC (Ser80Ile) were identified in one nalidixic acid- and ciprofloxacin-resistant isolate. Faecal E. coli isolates of healthy pets could be a reservoir of antimicrobial resistance genes. © 2007 Elsevier B.V. All rights reserved.