Glycopeptide-resistant Enterococcus faecium. Analysis of the resistance genotype, virulence and genetic lines [Enterococcus faecium resistente a glucopéptidos. Análisis del genotipo de resistencia, virulencia y líneas genéticas]

  1. López, M. 1
  2. Álvarez-Martínez, M.J. 23
  3. Marco, F. 23
  4. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universitat de Barcelona
    info

    Universitat de Barcelona

    Barcelona, España

    ROR https://ror.org/021018s57

  3. 3 Hospital Clinic Barcelona
    info

    Hospital Clinic Barcelona

    Barcelona, España

    ROR https://ror.org/02a2kzf50

Revista:
Enfermedades Infecciosas y Microbiologia Clinica

ISSN: 0213-005X

Año de publicación: 2013

Volumen: 31

Número: 1

Páginas: 10-14

Tipo: Artículo

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DOI: 10.1016/J.EIMC.2012.05.010 SCOPUS: 2-s2.0-84872308007 WoS: WOS:000313859700004 GOOGLE SCHOLAR

Otras publicaciones en: Enfermedades Infecciosas y Microbiologia Clinica

Resumen

Objective: The objective of this study was to analyse the genotypic characteristics of all Enterococcus isolates with acquired vancomycin resistance (VRE) recovered in the Hospital Clinic (Barcelona, Spain) in a period of three years and two months. Methods: All VRE isolated in the referred Hospital in the period January 2004-March 2007 were included in the study. The vancomycin resistance mechanism was investigated, as well as other antibiotic resistance mechanisms. Isolates were also typed by pulsed-field-gel-electrophoresis (PFGE) and multi-locus-sequence-typing (MLST). Results: Thirty-nine VRE were recovered, all being identified as E. faecium, representing 2% of total enterococci obtained in that period. Thirty-eight of them carried the vanA gene, and one isolate the vanB2 gene. The 39 VRE were classified into 13 different pulsotypes (A-M), with one main pulsotype, A, which included 13 isolates. The sequence type was identified by MLST in 24 VRE (with unrelated or closely-related PFGE patterns), and they were ascribed to the clonal complex CC17, but two classified as CC9. All VRE showed a multiresistance phenotype, including, in most cases ampicillin, ciprofloxacin, erythromycin, streptomycin, gentamicin, kanamycin and chloramphenicol, harbouring multiple antibiotic resistance genes. The presence of esp and/or hyl genes was identified in 37 VRE. Conclusion: All VRE, but one, showed the vanA genotype and they were mostly ascribed to the high-risk clonal complex CC17. © 2012 Elsevier España, S.L. All rights reserved.