Characterisation of CTX-M and SHV extended-spectrum beta-lactamases and associated resistance genes in Escherichia coli strains of food samples in Tunisia

  1. Jouini, A. 12
  2. Vinué, L. 1
  3. Slama, K.B. 2
  4. Sáenz, Y. 1
  5. Klibi, N. 2
  6. Hammami, S. 3
  7. Boudabous, A. 2
  8. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

  3. 3 Veterinary Research Institute, Tunis, Tunisia
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Revista:
Journal of Antimicrobial Chemotherapy

ISSN: 0305-7453

Año de publicación: 2007

Volumen: 60

Número: 5

Páginas: 1137-1141

Tipo: Artículo

DOI: 10.1093/JAC/DKM316 PMID: 17855726 SCOPUS: 2-s2.0-35448956941 WoS: WOS:000250682100033 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Antimicrobial Chemotherapy

Repositorio institucional: lock_openAcceso abierto Editor

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Resumen

Objectives: To assess the occurrence of extended-spectrum β-lactamases (ESBLs) in Escherichia coli isolates of faecal samples of animals (n = 40) and food samples (n = 38) obtained in Tunisia in 2006, and to characterize the type of ESBLs, their genetic environments and the associated resistance genes. Methods: Samples were inoculated in supplemented media (2 mg/L cefotaxime) for isolation of broad-spectrum cephalosporin-resistant E. coli isolates (one isolate/sample). ESBLs and their genetic environments as well as integrons and their gene cassette composition were characterized by PCR and sequencing. Results: ESBL-producing E. coli isolates were detected in 10 of the 38 food samples analysed (26%) and in none of the tested animal faecal samples. Genes found were as follows (number of isolates): blaCTX-M-1 (5), blaCTX-M-1 + blaTEM-1b (1), blaCTX-M-14 + blaTEM-1b (2), blaCTX-M-8 (1) and blaSHV-5 (1). All ESBL-positive isolates showed unrelated PFGE patterns. IS Ecp1 and IS 903 were detected surrounding blaCTX-M-14, and IS Ecp1/IS 26 and orf477 surrounding some of the blaCTX-M-1 genes. Four of the ESBL-positive strains harboured class 1 integrons including different gene cassette combinations. Conclusions: ESBLs, mainly of the CTX-M class, are detected in E. coli of food origin in Tunisia, being the first time that this mechanism has been detected in food E. coli strains in Africa. ©The Author 2007. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.