Detection and Molecular Characterization of Escherichia coli Strains Producers of Extended-Spectrum and CMY-2 Type Beta-Lactamases, Isolated from Turtles in Mexico

  1. Cortés-Cortés, G. 2
  2. Lozano-Zarain, P. 2
  3. Torres, C. 1
  4. Castañeda, M. 2
  5. Moreno Sánchez, Gabriela . 2
  6. Alonso, C.A. 1
  7. López-Pliego, Liliana . 2
  8. Mayen, M.G.G. 2
  9. Martínez-Laguna, Y. 2
  10. Del Carmen Rocha-Gracia, R. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Benemérita Universidad Autónoma de Puebla
    info

    Benemérita Universidad Autónoma de Puebla

    Heróica Puebla de Zaragoza, México

    ROR https://ror.org/03p2z7827

Revista:
Vector-Borne and Zoonotic Diseases

ISSN: 1530-3667

Año de publicación: 2016

Volumen: 16

Número: 9

Páginas: 595-603

Tipo: Artículo

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DOI: 10.1089/VBZ.2014.1725 SCOPUS: 2-s2.0-84984830349 WoS: WOS:000382281600005 GOOGLE SCHOLAR

Otras publicaciones en: Vector-Borne and Zoonotic Diseases

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Resumen

Multidrug-resistant bacteria are a growing problem in different environments and hosts, but scarce information exists about their prevalence in reptiles. The aim of this study was to analyze the resistance mechanisms, molecular typing, and plasmid content of cefotaxime-resistant (CTXR) Escherichia coli isolates recovered from cloacal samples of 71 turtles sheltered in a herpetarium in Mexico. CTXR-E. coli were recovered in 11 of 71 samples (15.5%), and one isolate/sample was characterized. Extended-spectrum β-lactamase (ESBL)-producing E. coli isolates were detected in four samples (5.6%): two strains carried the blaCTX-M-2 gene (phylogroup D and ST2732) and two contained the blaCTX-M-15 gene (phylogroup B1 and lineages ST58 and ST156). The blaCMY-2 gene was detected by PCR in E. coli isolates of eight samples (9.8%) (one of them also carried blaCTX-M-2); these isolates were distributed into phylogroups A (n = 1), B1 (n = 6), and D (n = 1) and typed as ST155, ST156, ST2329, and ST2732. Plasmid-mediated quinolone resistance (PMQR) genes were detected in five isolates [aac(6′)Ib-cr, qnrA, qnrB19, and oqxB]. From three to five replicon plasmids were detected among the strains, being IncFIB, IncI1, IncFrep, and IncK the most prevalent. ESBL or pAmpC genes were transferred by conjugation in four strains, and the blaCTX-M-15 and blaCMY-2 genes were localized in IncFIB or IncI1 plasmids by Southern blot hybridization assays. Class 1 and/or class 2 integrons were detected in eight strains with six different structures of gene cassette arrays. Nine pulsed-field gel electrophoresis patterns were found among the 11 studied strains. To our knowledge, this is the first detection of ESBL, CMY-2, PMQR, and mobile determinants of antimicrobial resistance in E. coli of turtle origin, highlighting the potential dissemination of multidrug-resistant bacteria from these animals to other environments and hosts, including humans. © 2016, Mary Ann Liebert, Inc.