First evidence of polar flagella in Klebsiella pneumoniae isolated from a patient with neonatal sepsis

  1. Carabarin-Lima, A. 2
  2. León-Izurieta, Libia. 2
  3. del Carmen Rocha-Gracia, R. 2
  4. Castañeda-Lucio, M. 2
  5. Torres, C. 1
  6. Gutiérrez-Cazarez, Z. 3
  7. González-Posos, Sirenia. 4
  8. Martínez de la Peña, C.F. 2
  9. Martinez-Laguna, Y. 2
  10. Lozano-Zarain, P. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Benemérita Universidad Autónoma de Puebla
    info

    Benemérita Universidad Autónoma de Puebla

    Heróica Puebla de Zaragoza, México

    ROR https://ror.org/03p2z7827

  3. 3 Laboratorio Clínico. Área de Microbiología, Hospital para el Niño Poblano, Blvd. del Niño Poblano No. 5307, Col. Concepción la Cruz, San Andrés, Cholula, Puebla, Mexico
  4. 4 Unidad de Microscopía Electrónica (LaNSE), CINVESTAV-IPN, Mexico
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Revista:
Journal of Medical Microbiology

ISSN: 0022-2615

Año de publicación: 2016

Volumen: 65

Número: 8

Páginas: 729-737

Tipo: Artículo

DOI: 10.1099/JMM.0.000291 SCOPUS: 2-s2.0-84986900851 WoS: WOS:000385014300004 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Medical Microbiology

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

The genus Klebsiella belongs to the family Enterobacteriaceae, and is currently considered to be non-motile and non-flagellated. In the present work, 25 Klebsiella strains isolated from nosocomial infections were assessed for motility under different growth conditions. One Klebsiella isolate, KpBUAP021, demonstrated a swim-like motility phenotype. The K. pneumoniae genotype was confirmed by 16S rRNA and rpoB gene sequence analysis. Multilocus sequence typing analysis also revealed that the KpBUAP021 strain places it in the ST345 sequence type, and belongs to the phylogenetic Kpl group. Transmission electron microscopy and the Ryu staining technique revealed that KpBUAP021 expresses polar flagella. Finally, the presence of fliC, fliA and flgH genes in this K. pneumoniae strain was confirmed. This report presents the first evidence for flagella-mediated motility in a K. pneumoniae clinical isolate, and represents an important finding related to its evolution and pathogenic potential. © 2016 The Authors.