Detection of CTX-M-15-Producing Escherichia coli Isolates of Lineages ST131-B2 and ST167-A in Environmental Samples of a Tunisian Hospital

  1. Dziri, R. 2
  2. Klibi, N. 2
  3. Alonso, C.A. 1
  4. Jouini, A. 3
  5. Ben Said, L. 2
  6. Chairat, S. 2
  7. Bellaaj, R. 4
  8. Boudabous, A. 2
  9. Ben Slama, K. 22
  10. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

  3. 3 Institut Pasteur de Tunis
    info

    Institut Pasteur de Tunis

    Túnez, Túnez

    ROR https://ror.org/04pwyer06

  4. 4 Service d'Hygiène Hospitalière et de Protection de l'Environnement, Hôpital Militaire de Tunis HMPIT, Montfleury, Tunisia
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Revista:
Microbial Drug Resistance

ISSN: 1076-6294

Año de publicación: 2016

Volumen: 22

Número: 5

Páginas: 399-403

Tipo: Artículo

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DOI: 10.1089/MDR.2015.0354 SCOPUS: 2-s2.0-84978252330 WoS: WOS:000379448400009 GOOGLE SCHOLAR

Otras publicaciones en: Microbial Drug Resistance

Resumen

To investigate the possible role of the hospital environment in the dissemination of extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli isolates, 300 samples were taken during 2013 from abiotic surfaces (n = 250), healthcare worker hands (n = 27), and hands of patients (n = 23) in a Tunisian Hospital. ESBL-producing E. coli isolates were recovered in 3.7% of analyzed samples (4% abiotic surfaces; 4.3% hands of patients; 0% in healthcare worker hands), and one isolate/sample was further studied. The characterization of beta-lactamase genes, as well as the genetic environment of blaCTX-M gene, was performed by PCR and sequencing. The ESBL genes found were as follows: blaCTX-M-15 (eight isolates), blaCTX-M-15+blaSHV-12 (two isolates), and blaSHV-12 (one isolate). The blaTEM-1b gene was detected in seven ESBL-positive isolates. The orf477 was found downstream of blaCTX-M-15 gene in 10 strains, whereas the ISEcp1 sequence was identified upstream of this gene in two isolates. The analysis of class 1 integrons by PCR and sequencing revealed five positive isolates with the following gene cassette arrangements: dfrA1-aadA1 (two isolates), aadA1 (two isolates), and aadA2 (one isolate). The virulence-encoding genes aer, eae, bfp, and hly were detected by PCR in six, four, four, and three isolates, respectively. The following sequence types and associated phylogroups were detected among ESBL-producing strains: ST167-phylogroup-A (six isolates) and ST131-phylogroup-B2 (two isolates). In conclusion, the hospital environment could be a reservoir of multiresistant bacteria, including ESBL-positive E. coli isolates, which could be acquired by the patient population, and strict control measures should be established to minimize this problem. © Copyright 2016, Mary Ann Liebert, Inc. 2016.