Genotypic and phenotypic characterization of methicillin-resistant Staphylococcus aureus (MRSA) clones with high-level mupirocin resistance

  1. González-Domínguez, M. 5
  2. Seral, C. 45
  3. Potel, C. 36
  4. Sáenz, Y. 3
  5. Álvarez, M. 36
  6. Torres, C. 12
  7. Castillo, F.J. 45
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Complexo Hospitalario Universitario de Vigo
    info

    Complexo Hospitalario Universitario de Vigo

    Vigo, España

    ROR https://ror.org/01ybfxd46

  4. 4 Universidad de Zaragoza
    info

    Universidad de Zaragoza

    Zaragoza, España

    ROR https://ror.org/012a91z28

  5. 5 Hospital Clínico Universitario Lozano Blesa
    info

    Hospital Clínico Universitario Lozano Blesa

    Zaragoza, España

    ROR https://ror.org/03fyv3102

  6. 6 Unidad de Apoyo a la Investigación, Instituto de Investigación Biomédica de Vigo (IBIV), Spain
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Revista:
Diagnostic Microbiology and Infectious Disease

ISSN: 0732-8893

Año de publicación: 2016

Volumen: 85

Número: 2

Páginas: 213-217

Tipo: Artículo

DOI: 10.1016/J.DIAGMICROBIO.2016.02.021 SCOPUS: 2-s2.0-84964292813 WoS: WOS:000376822600019 GOOGLE SCHOLAR

Otras publicaciones en: Diagnostic Microbiology and Infectious Disease

Resumen

A high proportion of methicillin-resistant Staphylococcus aureus isolates recovered in one year period showed high-level mupirocin-resistance (HLMUPR-MRSA) in our environment (27.2%). HLMUPR-MRSA isolates were mainly collected from skin and soft tissue samples, and diabetes was the main related comorbidity condition. These isolates were more frequently found in vascular surgery. HLMUPR-MRSA was more resistant to aminoglycosides than mupirocin-susceptible MRSA, linked to the presence of bifunctional and/or nucleotidyltransferase enzymes with/without macrolide resistance associated with the msr(A) gene. Most of HLMUPR-MRSA isolates belonged to ST125/t067. Nine IS257-ileS2 amplification patterns (p3 was the most frequent) were observed in HLMUPR-MRSA isolates, suggesting the presence of several mupirocin-resistance-carrying plasmids in our environment and promoting the emergence of mupirocin resistance. The presence of the same IS257-ileS2 amplification pattern p3 in 65% of HLMUPR-MRSA, all of them ST125/t067, suggests a clonal spread in our hospital and community environment which could explain the high prevalence of HLMUPR-MRSA during the study period. An outbreak situation or an increase in mupirocin consumption was not observed. © 2016 Elsevier Inc.