Detection of MRSA ST3061-t843-mecC and ST398-t011-mecA in white stork nestlings exposed to human residues

  1. Gómez, P. 1
  2. Lozano, C. 1
  3. Camacho, M.C. 2
  4. Lima-Barbero, J.-F. 2
  5. Hernández, José Manuel . 2
  6. Zarazaga, M. 1
  7. Höfle, U. 2
  8. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Instituto de Investigación en Recursos Cinegéticos
    info

    Instituto de Investigación en Recursos Cinegéticos

    Ciudad Real, España

    ROR https://ror.org/0140hpe71

Revista:
Journal of Antimicrobial Chemotherapy

ISSN: 0305-7453

Año de publicación: 2016

Volumen: 71

Número: 1

Páginas: 53-57

Tipo: Artículo

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DOI: 10.1093/JAC/DKV314 SCOPUS: 2-s2.0-84960113208 WoS: WOS:000371238100009 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Antimicrobial Chemotherapy

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Resumen

Objectives: The objective of this study was to analyse the prevalence of tracheal carriage of Staphylococcus aureus/MRSA in storks and to study the resistance and virulence genes in the obtained isolates. Methods: Tracheal samples from 92 stork nestlings of two landfill-associated and two natural-habitat colonies were inoculated in specific media for S. aureus and MRSA recovery. Antimicrobial susceptibility was tested, and the presence of resistance, virulence and immune evasion cluster (IEC) genes was analysed by PCR. S. aureus isolates were characterized by spa and agr typing. Staphylococcal cassette chromosome (SCC) mec type was determined for mecC-positive isolates, and MLST was performed for 17 selected S. aureus isolates. Results: S. aureus isolateswere identified in 32/92 samples (34.8%), and 38 isolateswere recovered. The prevalence of S. aureus was higher in nestlings from landfills (24/43, 55.8%) than in those fromnatural habitats (8/49, 16.3%). Three birds from landfill-associated colonies carried MRSA, two with mecA-positive strains [clonal complex (CC) 5-spa-t002 and CC398-spa-t011] and one with a mecC-positive strain [sequence type (ST) 3061-CC130-spat843- agr-III-SCCmecXI). None of the MRSA isolates presented IEC genes. Thirty-five MSSA isolates, which showed 18 different spa types (ascribed to CC5, CC7, CC22, CC30, CC45, CC59, CC133 and CC398), were obtained. The agr types detected were I (63%), II (29%) and III (8%). Resistance and virulence genes identified in MSSA were blaZ (n=25), erm(T) (n=9), erm(A) (n=1), tet(M) (n=2), fexA (n=3), str (n=2), tst (n=2), eta (n=1) and cna (n=15). The IEC types B, C, D and G were found in MSSA isolates, and two new STs were identified (ST3060 and ST3061). Conclusions: White storks are frequently tracheal carriers of S. aureus, including ST398 isolates. MRSA isolates of lineages CC398-mecA and CC130-mecC were detected in storks from landfill-associated colonies exposed to human residues. © The Author 2015. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.