Iberian Lynx (Lynx pardinus) from the captive breeding program as reservoir of antimicrobial resistant enterococci and Escherichia coli isolates

  1. Gonçalves, A. 2222
  2. Igrejas, G. 22
  3. Radhouani, H. 2222
  4. Santos, T. 2222
  5. Monteiro, R. 2222
  6. Marinho, C. 2222
  7. Pérez, M.J. 5
  8. Canales, R. 3
  9. Mendoza, J.L. 4
  10. Serra, R. 6
  11. Torres, C. 1
  12. Poeta, P. 22
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  3. 3 El Acebuche Captive Breeding Centre, Huelva, Spain
  4. 4 La Granadilla Breeding Centre, Zarza de Granadilla, Spain
  5. 5 La Olivilla Captive Breeding Centre, Santa Helena, Spain
  6. 6 National Centre for Captive Breeding of the Iberian Lynx, Silves, Portugal
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Revista:
Journal of Integrated OMICS

ISSN: 2182-0287

Año de publicación: 2013

Volumen: 3

Número: 2

Páginas: 138-144

Tipo: Artículo

DOI: 10.5584/JIOMICS.V3I2.142 SCOPUS: 2-s2.0-84897587979 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Journal of Integrated OMICS

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

A total of 98 faecal samples from captive specimens of Iberian lynx were collected and analysed for enterococci (96 isolates) and Escherichia coli (90 isolates) recovery. These 186 isolates were tested for antimicrobial resistance, molecular mechanisms of resistance, and detection of virulence genes. Among the enterococci, Enterococcus hirae was the most prevalent species (35 isolates), followed by Enterococcus faecalis (30 isolates), Enterococcus faecium (27 isolates), and Enterococcus durans (4 isolates). High rates of resistance to tetracycline, erythromycin and high-level-kanamycin were detected among enterococcal isolates (41%, 26%, and 19%, respectively). The tet(M) and/or tet(L), erm(B), aac(6′)-Ie-aph(2″)-Ia, ant(6)-Ia, or aph(3′)-IIIa genes were detected among resistant enterococci. Likewise, high rates of resistance were detected in E. coli isolates to tetracycline, streptomycin, sulfamethoxazole-trimethoprim (SXT), nalidixic acid, ampicillin, and ciprofloxacin (34%, 28%, 26%, 21%, 17%, and 16%, respectively). Furthermore, the blaTEM or blaSHV, tet(A) and/or tet(B), aadA or strA-strB, aac(3)-II and/or aac(3)-IV, and different combinations of sul genes were detected among most resistant isolates. Fifteen isolates contained class 1 and/or class 2 integrons and 3 different gene cassette arrays were identified (aadA1, dfrA1+aadA1, and estX+psp+aadA2). The E. coli isolates were ascribed to phylo-groups A (12%); B1 (40%); B2 (37%), and D (11%), being fimA the most prevalent virulence gene (n=84), followed by aer (n=13), cnf1 (n=13), papC (n=10) and papG-allele III (n=9). This study showed specimens of Iberian lynx acting as reservoirs of resistance genes, and in future (re)introductions they could spread resistant bacteria throughout the environment