Comparative proteomic map among vanA-containing Enterococcus isolated from yellow-legged gulls

  1. Radhouani, H. 4
  2. Poeta, P. 24
  3. Pinto, L. 4
  4. Monteiro, R. 24
  5. Nunes-Miranda, J. 4
  6. Correia, S. 24
  7. Vieira, S. 24
  8. Carvalho, C. 4
  9. Rodrigues, J. 24
  10. López, M. 1
  11. Torres, C. 1
  12. Vitorino, R. 3
  13. Domingues, P. 3
  14. Igrejas, G. 4
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Center of Studies of Animal and Veterinary Sciences, Vila Real, Portugal
  3. 3 Universidade de Aveiro
    info

    Universidade de Aveiro

    Aveiro, Portugal

    ROR https://ror.org/00nt41z93

  4. 4 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

Mostrar afiliaciones +
Revista:
Journal of Integrated OMICS

ISSN: 2182-0287

Año de publicación: 2012

Volumen: 2

Número: 1

Páginas: 46-54

Tipo: Artículo

DOI: 10.5584/JIOMICS.V2I1.86 SCOPUS: 2-s2.0-85038615556 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Journal of Integrated OMICS

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

The increase of VRE, therefore, represents an urgent threat for patient care and creates a reservoir of mobile resistance genes for other, more virulent pathogens. The existence of VRE in different ecologic niches complicates the understanding of its epidemiology. The aim of the present study was to study the proteome of 2 vanA strains recovered from seagull faecal samples. The vanA E. durans and vanA E. faecium isolates presented different genomic patterns: tet(M)-tet(L)-erm(B) and tet(M)-tet(L)-erm(B)-hyl, respectively. A total of 123 spots were excised from two-dimensional gel electrophoresis (2-DE) gel of vanA E. durans SG 2 strain, and 16 were successfully identified by using MS, representing 42 different proteins. For the vanA E. faecium SG 50 strain, 93 spots were excised from the 2-DE gel and 23 were identified, representing 47 different proteins. The vancomycin/teicoplanin A-type resistance protein vanA in vanA E. durans SG 2 strain was present in two different spots. The identified proteins have shown diverse functional activities including glycolysis, conjugation, translation, protein biosynthesis, among others. This work reports the impact of proteomics on knowledge of vanA enterococci strains and will be helpful to further understand the antibiotic-resistant mechanism.