Characterization of Staphylococcus spp strains isolated from hospital, community and environmental in Puebla City, Mexico

  1. de los Ángeles Lobo-Sánchez, A.M. 2
  2. Lozano-Zaraín, P. 2
  3. Martínez-Laguna, Y. 22
  4. Gutiérrez-Cázarez, Z. 3
  5. Rivera-Tapia, A. 2
  6. Torres, C. 1
  7. del Carmen Rocha-Gracia, R. 22
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Benemérita Universidad Autónoma de Puebla
    info

    Benemérita Universidad Autónoma de Puebla

    Heróica Puebla de Zaragoza, México

    ROR https://ror.org/03p2z7827

  3. 3 Hospital para el Niño Poblano, Blvd. del Nino Poblano No. 5207 Col Concepcion la Cruz, 72190 Puebla, Pue, Mexico
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Revista:
African Journal of Microbiology Research

ISSN: 1996-0808

Año de publicación: 2011

Volumen: 5

Número: 16

Páginas: 2266-2272

Tipo: Artículo

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SCOPUS: 2-s2.0-84856502126 WoS: WOS:000298918200013 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: African Journal of Microbiology Research

Resumen

We investigated the prevalence of methicillin-resistant staphylococci (MRS) and evaluated the antimicrobial resistance patterns of 284 Staphylococcus strains isolated from two hospitals, as well as from community and the environment in Puebla City, Mexico. Isolates were identified by Gram-stain and biochemical reactions and antimicrobial susceptibility testing was performed by Kirby-Bauer and in some cases by determination of minimal inhibitory concentrations of antimicrobial agents. Result showed that from 284 strains studied, 32% (90 strains) were multidrug-resistant, of which 82% (74 strains) were methicillin-resistant. From 154 Staphylococcus aureus isolates tested, 41 strains showed methicillin-resistance (27%) and 84 of 130 coagulase-negative staphylococci showed methicillin-resistance (65%). The mecA gene was detected in 38 of 43 oxacillin-resistant staphylococci tested (14/14 S. aureus and 24/29 coagulase negative staphylococci). Nevertheless, this gene was also identified in 14 S. aureus that exhibited oxacillin and cefoxitin susceptibility. The percentages of resistance detected among S. aureus and coagulase negative staphylococcal isolates were: penicillin (79 and 67%, respectively), oxacillin (27 and 65%), erythromycin (18 and 36%), tetracycline (6 and 24%), gentamicin (71 and 45%), and trimethoprim sulfamethoxazole (19 and 37%). The β-lactamase production was positive in more than 80% of isolates. These results show the presence of multiresistant strains in these three sources, which supports the control measures taken by health authorities with respect to avoiding the misuse and abuse of antibiotics. © 2011 Academic Journals.