Molecular detection and characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolates from dogs in Portugal

  1. Coelho, C. 2333
  2. Torres, C. 1
  3. Radhouani, H. 2333
  4. Pinto, L. 2333
  5. Lozano, C. 1
  6. Gómez-Sanz, E. 1
  7. Zaragaza, M. 1
  8. Igrejas, G. 33
  9. Poeta, P. 13
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Center of Studies of Animal and Veterinary Sciences, Vila Real, Portugal
  3. 3 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

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Revista:
Microbial Drug Resistance

ISSN: 1076-6294

Año de publicación: 2011

Volumen: 17

Número: 2

Páginas: 333-337

Tipo: Artículo

DOI: 10.1089/MDR.2010.0080 PMID: 21254810 SCOPUS: 2-s2.0-79957647003 WoS: WOS:000291001100027 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Microbial Drug Resistance

Resumen

Fifty-four healthy dogs were screened in Portugal for the presence of nasal methicillin-resistant Staphylococcus aureus (MRSA) carriage. Sixteen MRSA isolates (one/sample) were recovered from nasal samples of dogs, and they were typed by molecular methods (S. aureus protein A [spa]-, multilocus sequence typing-, staphylococcal cassette chromosome mec-typing). MRSA isolates were investigated for their susceptibility to antimicrobial agents by disk-diffusion test. The presence of resistance genes and of the Panton-Valentine leukocidin gene (lukF-lukS) was analyzed by PCR. Four different spa-types were identified among our MRSA isolates (t032, t432, t747, and t4726), with t032 as the most frequently detected. The sequence-type ST22 was identified in four tested MRSA isolates with different spa-types. All 16 isolates presented the staphylococcal cassette chromosome mec type IV. Most of MRSA isolates were resistant to ciprofloxacin, erythromycin, and clindamycin (94%-100%), and no resistance was identified to chloramphenicol, mupirocin, and trimethoprim-sulfametoxazole. The ermC and tetM resistance genes were detected in all MRSA isolates. The amino acid changes Ser84Leu in GyrA protein and Ser80Phe in GrlA protein were the most prevalent ones in our MRSA isolates. None of the MRSA strains carried the lukF-lukS genes. The results presented in this study indicate that healthy dogs may be a reservoir of MRSA that could be transmitted to humans by direct contact. © 2011, Mary Ann Liebert, Inc.