Genetic detection and multilocus sequence typing of vanA-containing enterococcus strains from mullets fish (Liza ramada)

  1. Araújo, C. 2222
  2. Torres, C. 1
  3. Gonçalves, A. 2222
  4. Carneiro, C. 2
  5. López, M. 1
  6. Radhouani, H. 2222
  7. Pardal, M. 3
  8. Igrejas, G. 22
  9. Poeta, P. 22
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  3. 3 Universidade de Coimbra
    info

    Universidade de Coimbra

    Coímbra, Portugal

    ROR https://ror.org/04z8k9a98

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Revista:
Microbial Drug Resistance

ISSN: 1076-6294

Año de publicación: 2011

Volumen: 17

Número: 3

Páginas: 357-361

Tipo: Artículo

DOI: 10.1089/MDR.2010.0171 SCOPUS: 2-s2.0-80052213095 WoS: WOS:000294772600004 GOOGLE SCHOLAR

Otras publicaciones en: Microbial Drug Resistance

Resumen

Enterococci have emerged as important nosocomial and community-acquired pathogens in humans. The presence of vanA-enterococci was investigated in 103 fecal samples recovered from mullets fish (Liza ramada). All fecal samples were inoculated in Slanetz-Bartley agar plates supplemented with 4mg/L of vancomycin for vancomycin-resistant enterococci (VRE) recovery and two isolates/sample were characterized. Antibiotic susceptibility was tested for 11 antibiotics by disk diffusion and agar dilution methods. VRE identification was performed by biochemical and molecular methods. Additionally, the mechanisms of resistance to glycopeptides (vanA, vanB, vanC1, vanC2, and vanD) and other antibiotics [erm(A), erm(B), tet(L), tet(M), aph(2′′)-aac(6′), aph(3′)-IIIa, ant(6′), vat(D), vat(E)] as well as the presence of enterococcal surface protein (esp) and hyl virulence factors were investigated. vanA-Enterococcus faecium isolates were recovered from 4 of 103 tested samples, and they showed glycopeptide and erythromycin resistances. Three of them were also ampicillin resistant, two showed resistance to tetracycline, ciprofloxacin, and kanamycin, and one showed resistance to gentamicin. The tet(M) and erm(B) genes were found in all tetracycline- and erythromycin-resistant strains, respectively. The aph(3′)-III and aph(2′′)-aac(6′) genes were identified in the kanamycin- and gentamicin-resistant isolates, respectively. The IS1216 element was identified within vanX-vanY region of Tn1546 in two vanA isolates. The hyl and esp virulence genes were found in four and two isolates, respectively. vanA-strains were ascribed to sequence types ST280 (two isolates) and ST273 (two isolates), including both lineages into the clonal complex CC17. Mullets fish can excrete VRE in their feces and may be a reservoir for such resistant bacteria that can be transmitted to other animals including humans. © 2011, Mary Ann Liebert, Inc.