Apparent Expression of Small Single Copy cpDNA Region and Respiration in Senescent Chloroplasts

  1. Vera, A. 1
  2. Tomás, R. 1
  3. Martín, M. 1
  4. Sabater, B. 1
  1. 1 Universidad de Alcalá
    info

    Universidad de Alcalá

    Alcalá de Henares, España

    ROR https://ror.org/04pmn0e78

Revista:
Plant Science

ISSN: 0168-9452

Año de publicación: 1990

Volumen: 72

Número: 1

Páginas: 63-67

Tipo: Artículo

SCOPUS: 2-s2.0-38249021145 GOOGLE SCHOLAR

Otras publicaciones en: Plant Science

Resumen

Tobacco dpDNA fragments have been used as probes for hybrid select translation in a protein synthesis system (derived from nuclease-treated senescent barley chloroplasts) depending on externally added mRNA retaining translational specificity (as it preferently translated mRNAs for polypeptides synthesized during senescence). Selections from crude mRNAs (isolated from senescent chloroplasts containing some mRNAs not translated in vivo) and translations indicated that most genes for the polypeptides synthesized early in leaf senescence map in the SSC cpDNA region. This region is poorly expressed during chloroplast biogenesis and includes four genes (ndh) homologous to those for components of mitochondrial NADH dehydrogenase. Accordingly, senescent chloroplasts show high ferricyanide reducing activity, probably related to NADH dehydrogenase. Apparently, chloroplast ndh gene expression is activated during leaf senescence at the level of translation. © 1990.