Preparation of Chloroplast DNA from Barley and Lettuce and Comparison of Restriction Fragments

  1. Martin, M. 1
  2. Tomás, R. 1
  3. Cruz Izquierdo, M. 1
  4. Prádanos, C. 1
  5. Sabater, B. 1
  1. 1 Universidad de Alcalá
    info

    Universidad de Alcalá

    Alcalá de Henares, España

    ROR https://ror.org/04pmn0e78

Revista:
Biologia plantarum

ISSN: 0006-3134

Año de publicación: 1989

Volumen: 31

Número: 1

Páginas: 67-71

Tipo: Artículo

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DOI: 10.1007/BF02890680 SCOPUS: 2-s2.0-51849182859 GOOGLE SCHOLAR

Otras publicaciones en: Biologia plantarum

Resumen

Chloroplast DNA from three barley cultivars and from one lettuce cultivar was prepared from chloroplasts isolated by Conventional differential centrifugation. Barley chloroplast DNA size was sensibly lower (130 kpb) than lettuce chloroplast DNA (150 kpb). Chloroplast DNAs from the three barley cultivars showed similar restriction fragment patterns after digestion with: BamHI, EcoRI or HindIII. The lettuce chloroplast DNA restriction pattern was very different from the barley chloroplast DNA restriction pattern. © 1989 Academia.