Detection of multiple-antimicrobial resistance and characterization of the implicated genes in Escherichia coli isolates from foods of animal origin in Tunis

  1. Jouini, A. 12
  2. Slama, K.B. 2
  3. Sáenz, Y. 1
  4. Klibi, N. 2
  5. Costa, D. 1
  6. Vinué, L. 1
  7. Zarazaga, M. 1
  8. Boudabous, A. 2
  9. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

Revista:
Journal of Food Protection.

ISSN: 0362-028X

Año de publicación: 2009

Volumen: 72

Número: 5

Páginas: 1082-1088

Tipo: Artículo

DOI: 10.4315/0362-028X-72.5.1082 PMID: 19517738 SCOPUS: 2-s2.0-66949173382 WoS: WOS:000265955600023 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Food Protection.

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

Phenotypic and genotypic characterization of antimicrobial resistance was conducted for 98 Escherichia coli isolates recovered from 40 food samples of animal origin (poultry, sheep, beef, fish, and others) obtained in supermarkets and local butcheries in Tunis during 2004 and 2005. Susceptibility to 15 antimicrobial agents was tested by disk diffusion and agar dilution methods, the mechanisms of resistance were evaluated using PCR and sequencing methods, and the clonal relationship among isolates was evaluated using pulsed-field gel electrophoresis. High resistance was detected to tetracycline, sulphonamides, nalidixic acid, ampicillin, streptomycin, and trimethoprim-sulfamethoxazole (29 to 43% of isolates), but all isolates were susceptible to cefotaxime, ceftazidime, cefoxitin, azthreonam, and amikacin. One-third of the isolates had multiresistant phenotypes (resistance to at least five different families of antimicrobial agents). Different variants of blaTEM, tet, sul, dfrA, aadA, and aac(3) genes were detected in most of the strains resistant to ampicillin, tetracycline, sulphonamide, trimethoprim, streptomycin, and gentamicin, respectively. The presence of class 1 and class 2 integrons was studied in 15 sulphonamideresistant unrelated E. coli strains, and 14 of these strains harbored class 1 integrons with five different arrangements of gene cassettes, and a class 2 integron with the dfrAl + sat + aadAl arrangement was found in one strain. This study revealed the high diversity of antimicrobial resistance genes, some of them included in integrons, in E. coli isolates of food origin. Copyright ©, International Association tor Food Protection.