Genetic lineages and antimicrobial resistance genotypes in Staphylococcus aureus from children with atopic dermatitis: detection of clonal complexes CC1, CC97 and CC398

  1. Benito, D. 2
  2. Aspiroz, C. 5
  3. Gilaberte, Y. 14
  4. Sanmartín, R. 14
  5. Hernández-Martin, Á. 3
  6. Alonso, M. 3
  7. Gómez, P. 2
  8. Lozano, C. 2
  9. Torres, C. 2
  1. 1 Instituto de Investigación Sanitaria Aragón
    info

    Instituto de Investigación Sanitaria Aragón

    Zaragoza, España

  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  3. 3 Hospital Infantil Universitario Niño Jesus de Madrid
    info

    Hospital Infantil Universitario Niño Jesus de Madrid

    Madrid, España

    ROR https://ror.org/028brk668

  4. 4 Department of Dermatology, Hospital San Jorge, Huesca, Spain
  5. 5 Department of Microbiology, Hospital Royo Villanova, Zaragoza, Spain
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Revista:
Journal of Chemotherapy

ISSN: 1120-009X

Año de publicación: 2016

Volumen: 28

Número: 5

Páginas: 359-366

Tipo: Artículo

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DOI: 10.1179/1973947815Y.0000000044 SCOPUS: 2-s2.0-84979497669 WoS: WOS:000384332700002 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Chemotherapy

Objetivos de desarrollo sostenible

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Resistencia bacteriana a antimicrobianos en ambiente y fauna salvaje. Mecanismos de resistencia y líneas genéticas emergentes e implicaciones en salud humana

2013/00001/001

Resumen

The objective was to analyse the genetic lineages of Staphylococcus aureus recovered from nasal and skin samples of atopic dermatitis (AD) paediatric patients, and to characterize the antimicrobial resistance phenotype-genotype and the immune-evasion-cluster (IEC) type of isolates. Forty S. aureus isolates from 35 patients (skin: 26; nasal samples: 14) were characterized. Isolates were submitted to spa-, agr- and multilocus sequence typing. All S. aureus strains analyzed were methicillin-susceptible (MSSA). High genetic diversity was detected among the 40 MSSA isolates (especially among skin isolates), with detection of 27 different spa-types, 20 sequence-types and 16 clonal complexes (CCs). Lineages CC30 and CC5 were predominant among nasal isolates (71% vs 23% skin). Thirteen different CCs were detected among skin isolates, with detection of clades CC1, CC9 and CC398. Antimicrobial resistance rates detected were higher in skin than in nasal isolates, especially for macrolides, aminoglycosides, lincosamides and mupirocin. Methicillin-susceptible S. aureus strains were characterized into five IEC-types, being A, B and F the predominant ones. Methicillin-susceptible S. aureus strains of lineages CC45 and CC5 were detected in almost all cases in AD patients with severe Scoring Atopic Dermatitis (SCORAD) and lineages CC8, and CC30 in those with mild or moderate one. As conclusion, high-clonal-diversity was detected among MSSA from AD patients, especially in skin-isolates. Colonization with S. aureus of some CCs seems more associated with AD severity than other lineages.