Clonal lineages detected amongst tetracyclineresistant meticillin-resistant Staphylococcus aureus isolates of a Tunisian hospital, with detection of lineage ST398

  1. Elhani, D. 4
  2. Gharsa, H. 2
  3. Kalai, D. 3
  4. Lozano, C. 1
  5. Gómez, P. 1
  6. Boutheina, J. 3
  7. Aouni, M. 4
  8. Barguellil, F. 3
  9. Torres, C. 1
  10. Slama, K.B. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Université de Tunis El Manar
    info

    Université de Tunis El Manar

    Túnez, Túnez

    ROR https://ror.org/029cgt552

  3. 3 Military Hospital of Tunis
    info

    Military Hospital of Tunis

    Túnez, Túnez

    ROR https://ror.org/04n4f3r80

  4. 4 University of Monastir
    info

    University of Monastir

    Monastir, Túnez

    ROR https://ror.org/00nhtcg76

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Revista:
Journal of Medical Microbiology

ISSN: 0022-2615

Año de publicación: 2015

Volumen: 64

Número: 6

Páginas: 623-629

Tipo: Artículo

DOI: 10.1099/JMM.0.000066 SCOPUS: 2-s2.0-84935139715 WoS: WOS:000359614000006 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Medical Microbiology

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

Tetracycline resistance has been postulated as a potential phenotypic marker of livestockassociated lineage ST398 amongst meticillin-resistant Staphylococcus aureus (MRSA) clinical isolates in some European hospitals. The objective of this study was to determine if this marker could also be applied to Maghrebian countries. In total, 99 MRSA isolates were collected in a Tunisian hospital during January 2011–October 2012, and 24 tetracycline-resistant MRSA isolates of this collection were characterized. All isolates were tested for antimicrobial resistance phenotypes and genotypes, molecular typing, and virulence genes. Multilocus sequence typing showed that the majority of the isolates (19/24) belonged to clonal complex CC8 (ST247, n512 isolates; ST239, n56isolates; ST241, n51 isolate). The remaining isolates belonged to CC398 (ST398, n51 isolate), CC5 (ST5 and ST641, n52 isolates), and CC80 (ST728, n52 isolates). Spa typing discriminated MRSA in eight spa types: t052 (n512 isolates), t037 (n55 isolates), t044 (n52 isolates), and t899, t129, t311, t1744 and the new t14712 (n51 isolate each). Three agr groups were found amongst the studied isolates: agr group I (n520 isolates), agr group II (n52) and agr group III (n52 isolates). We report the detection of one MRSA ST398–t899 isolate in the nasal sample of a farmer patient in Tunisia, representing the first report of ST398 in humans in Africa. Tetracycline resistance seems not to be a good phenotypic marker for MRSA ST398 strains in Tunisia, where CC8 was the most prevalent lineage. Continuous efforts to understand the changing epidemiology of this micro-organism are necessary not only for appropriate antimicrobial treatment and effective infection control, but also to monitor its evolution. © 2015 The Authors.