Tn1546 structures and Multilocus-Sequence-Typing (MLST) of vanA-containing enterococci of animal, human and food origin.

  1. López, M. 1
  2. Sáenz, Y. 2
  3. Álvarez-Martínez, M.J. 3
  4. Marco, F. 3
  5. Robredo, B. 1
  6. Rojo-Bezares, B. 2
  7. Ruiz-Larrea, F. 1
  8. Zarazaga, M. 1
  9. Torres, C. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Centro de Investigación Biomédica de La Rioja
    info

    Centro de Investigación Biomédica de La Rioja

    Logroño, España

    ROR https://ror.org/03vfjzd38

  3. 3 Institut d'Investigacions Biomèdiques August Pi i Sunyer
    info

    Institut d'Investigacions Biomèdiques August Pi i Sunyer

    Barcelona, España

    ROR https://ror.org/054vayn55

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Revista:
Journal of Antimicrobial Chemotherapy

ISSN: 0305-7453

Año de publicación: 2010

Volumen: 65

Número: 8

Páginas: 1570-1575

Tipo: Artículo

DOI: 10.1093/JAC/DKQ192 PMID: 20519356 SCOPUS: 2-s2.0-77954732503 WoS: WOS:000280018400004 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Antimicrobial Chemotherapy

Resumen

Objectives: To characterize the Tn1546 structure and to perform the genetic typing of 51 PFGE-unrelated vanAcontaining enterococci of different origin (clinical, food and faecal samples of healthy humans and healthy poultry). Methods: Tn1546 structure was characterized by a PCR primer walking strategy and sequencing. Multilocus sequence typing (MLST) was performed for Enterococcus faecium and Enterococcus faecalis strains, and esp and hyl genes were detected by PCR. Results: Nine different Tn1546 structures were identified in the studied strains. Type I was the most prevalent structure (75%) (identical to GenBank M97297). Two new Tn1546 structures were identified (in three clinical and animal strains), containing two new insertion sequences (ISs; ISEfa11 disrupting vanS and ISEfa10 disrupting orf1). An additional new Tn1546 structure was found in one animal strain, containing ISEf1 interrupting vanY and IS1542 in the orf2-vanR region. A high diversity of sequence types (STs) was detected among clinical (6 ST/18 strains) and non-clinical E. faecium strains (18 ST/24 strains). STs associated with clonal complexes CC17 and CC9 were mainly detected among clinical and non-clinical E. faecium strains, respectively. Seven new STs were identified in non-clinical strains. The esp and hyl genes were only found among clinical E. faecium strains. Conclusions: A moderate variability in Tn1546 structure has been detected among unrelated vanA-containing enterococci of different origins, showing three new structures including two new ISs. A high diversity of STs was detected among E. faecium strains, especially among non-clinical strains, and new STs have been identified. © The Author 2010.