First report on MRSA CC398 recovered from wild boars in the north of Portugal. Are we facing a problem?

  1. Sousa, M. 236
  2. Silva, N. 5
  3. Manageiro, V. 16
  4. Ramos, S. 2
  5. Coelho, A. 4
  6. Gonçalves, D. 1
  7. Caniça, M. 16
  8. Torres, C. 3
  9. Igrejas, G. 137
  10. Poeta, P. 127
  1. 1 Universidade Do Porto
    info

    Universidade Do Porto

    Oporto, Portugal

    ROR https://ror.org/043pwc612

  2. 2 Universidade de Trás-os-Montes e Alto Douro
    info

    Universidade de Trás-os-Montes e Alto Douro

    Vila Real, Portugal

    ROR https://ror.org/03qc8vh97

  3. 3 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  4. 4 Forest Association of Trás-os-Montes and Alto Douro (AFTM), Vila Real, Portugal
  5. 5 Moredun Research Institute, Pentlands Science Park, Penicuik, Scotland, United Kingdom
  6. 6 National Reference Laboratory of Antibiotic Resistances and Healthcare Associated Infections (NRL-AR/HAI), National Institute of Health Doutor Ricardo Jorge (NIH), Lisboa, Portugal
  7. 7 Universidade Nova de Lisboa
    info

    Universidade Nova de Lisboa

    Lisboa, Portugal

    ROR https://ror.org/02xankh89

Revista:
Science of The Total Environment

ISSN: 0048-9697

Año de publicación: 2017

Volumen: 596-597

Páginas: 26-31

Tipo: Artículo

DOI: 10.1016/J.SCITOTENV.2017.04.054 SCOPUS: 2-s2.0-85017430801 WoS: WOS:000401557600004 GOOGLE SCHOLAR

Otras publicaciones en: Science of The Total Environment

Resumen

The aim of the present study was to evaluate the resistance of Staphylococcus aureus recovered from wild boars, to analyze their genetic lineages, and to investigate the susceptibility to oxacillin. Samples from mouth and nose of 45 wild boars (Sus scrofa) were collected during hunt activity from November 2012 to January 2013 in the North of Portugal. S. aureus isolates were recovered from 30 of these samples (33%); one isolate/sample was further studied. The susceptibility of the isolates was tested by disk-diffusion test against 14 antimicrobial agents and minimal inhibitory concentration was used to test oxacillin according to EUCAST guidelines. The genetic lineages of S. aureus were characterized by agr-typing, spa-typing and MLST. From the 30 isolates, 18 S. aureus were susceptible to all antibiotics tested and 7 presented resistance to one or more of the following antibiotics: penicillin (n = 3), oxacillin (n = 4), cefoxitin (n = 1), clindamycin (n = 2), gentamicin (n = 1), fusidic acid (n = 1), ciprofloxacin (n = 2), tetracycline (n = 1) and linezolid (n = 1). One MRSA CC398 (spa-type t899) isolate was detected (oxacillin MIC = 32 mg/L and mecA-positive), which presented resistance to penicillin, tetracycline, and ciprofloxacin and contained the genes of immune evasion cluster (IEC) system (type B). The 29 methicillin-susceptible isolates were typed as ST1 (t1533), ST133 (t3583), ST1643 (t10712), ST2328 (t3750) and the new STs (3220, 3222, 3223, 3224) associated to new spa-types t14311 and t14312. The agr-types I, II, III and IV were identified. It is a matter of concern when MRSA and some specific lineages of S. aureus are taken as commensal habitants of the skin and nose of wild animals and are characterized with resistance to various antimicrobial agents in clinical use. © 2017