Analysis of blaSHV-12-carrying Escherichia coli clones and plasmids from human, animal and food sources

  1. Alonso, C.A. 2
  2. Michael, G.B. 36
  3. Li, J. 16
  4. Somalo, S. 2
  5. Simón, C. 4
  6. Wang, Y. 1
  7. Kaspar, H. 5
  8. Kadlec, K. 6
  9. Torres, C. 2
  10. Schwarz, S. 36
  1. 1 China Agricultural University
    info

    China Agricultural University

    Haidian, China

    ROR https://ror.org/04v3ywz14

  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  3. 3 Free University of Berlin
    info

    Free University of Berlin

    Berlín, Alemania

    ROR https://ror.org/046ak2485

  4. 4 Universidad de Zaragoza
    info

    Universidad de Zaragoza

    Zaragoza, España

    ROR https://ror.org/012a91z28

  5. 5 Federal Office of Consumer Protection and Food Safety (BVL), Berlin, Germany
  6. 6 Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut (FLI), Neustadt-Mariensee, Germany
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Revista:
Journal of Antimicrobial Chemotherapy

ISSN: 0305-7453

Año de publicación: 2017

Volumen: 72

Número: 6

Páginas: 1589-1596

Tipo: Artículo

DOI: 10.1093/JAC/DKX024 SCOPUS: 2-s2.0-85027383329 WoS: WOS:000404899800006 GOOGLE SCHOLAR

Otras publicaciones en: Journal of Antimicrobial Chemotherapy

Repositorio institucional: lock_openAcceso abierto Postprint

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Resumen

Objectives: This study aimed at characterizing 23 Escherichia coli isolates from various sources and their respective blaSHV-12-carrying plasmids and sequencing one of these plasmids completely. Methods: Isolates were typed by XbaI-PFGE, MLST and PCR-based phylotyping. Transformed blaSHV-12-carrying plasmids were examined by replicon typing, S1-nuclease, conjugation, EcoRI-HindIII-BamHI digests and plasmid MLST. Co-located resistance genes and integrons as well as the blaSHV-12 genetic environment were analysed by PCR and sequencing. One IncI1 plasmid was sequenced completely using HiSeq 2500 and gap closure by PCRs and Sanger sequencing. Results: Among the 23 SHV-12-positive E. coli, some isolates from different sources showed the same characteristics: ST23/phylogroup A (human, dog, livestock), ST57/D (wild bird, chicken meat) and ST117/D (chicken meat, chicken). All blaSHV-12 genes were horizontally transferable via 30-120 kb plasmids of incompatibility groups IncI1 (n=17), IncK (n=3), IncF (n=1), IncX3 (n=1) and a non-typeable plasmid. IncK plasmids, indistinguishable in size and restriction patterns, were found in isolates from different sources (ST57/D, meat; ST131/B2, meat; ST57/B1, dog). The IncI1-blaSHV-12-carrying plasmids were mostly assigned to plasmid ST (pST) 26 and pST3. Three plasmids showed novel pSTs (pST214, pST215). The majority of the IncI1 transformants exhibited resistance to β-lactams, chloramphenicol and streptomycin (in relation with a class 1 integron containing an estX-psp-aadA2-cmlA1-aadA1-qacI gene cassette array), and to tetracycline. A novel blaSHV-12 environment was detected and whole plasmid sequencing revealed a Tn21-derived-blaSHV12-ΔTn1721 resistance complex. Conclusions: Results from this study suggest that the dissemination of blaSHV-12 genes occurs by vertical (clonal) and horizontal transfer, the latter mainly mediated through IncI1 multidrug-resistance plasmids. © The Author 2017. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved.