Rapid Identification of Enterococcus hirae and Enterococcus durans by PCR and Detection of a Homologue of the E. hirae muramidase-2 Gene in E. durans
- Arias, C.A. 13
- Robredo, B. 2
- Singh, K.V. 1
- Torres, C. 2
- Panesso, D. 3
- Murray, B.E. 1
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1
University of Texas Health Science Center at Houston
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University of Texas Health Science Center at Houston
Houston, Estados Unidos
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2
Universidad de La Rioja
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- 3 Bacterial Molecular Genetics Unit, Universidad El Basque, Transv 9a Bis No. 133-25, Bogotá, D.C., Colombia
ISSN: 0095-1137
Año de publicación: 2006
Volumen: 44
Número: 4
Páginas: 1567-1570
Tipo: Artículo
Otras publicaciones en: Journal of Clinical Microbiology
Resumen
During an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2ed. PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strains only. PCR (under high-stringency conditions) with primers for the mur-2ed gene gave the expected amplification product only with E. durans strains. A combination of murG and mur-2ed primers in a multiplex PCR assay differentiated E. hirae from E. durans in all cases. PCR using these primers appears to be a rapid alternative for identification of E. hirae and E. durans isolates. Copyright © 2006, American Society for Microbiology. All Rights Reserved.