Rapid Identification of Enterococcus hirae and Enterococcus durans by PCR and Detection of a Homologue of the E. hirae muramidase-2 Gene in E. durans

  1. Arias, C.A. 13
  2. Robredo, B. 2
  3. Singh, K.V. 1
  4. Torres, C. 2
  5. Panesso, D. 3
  6. Murray, B.E. 1
  1. 1 University of Texas Health Science Center at Houston
    info

    University of Texas Health Science Center at Houston

    Houston, Estados Unidos

    GRID grid.267308.8

  2. 2 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    GRID grid.119021.a

  3. 3 Bacterial Molecular Genetics Unit, Universidad El Basque, Transv 9a Bis No. 133-25, Bogotá, D.C., Colombia
Journal:
Journal of Clinical Microbiology

ISSN: 0095-1137

Year of publication: 2006

Volume: 44

Issue: 4

Pages: 1567-1570

Type: Article

Export: RIS
DOI: 10.1128/JCM.44.4.1567-1570.2006 PMID: 16597896 SCOPUS: 2-s2.0-33645924366 WoS: 000236810500057 GOOGLE SCHOLAR

Abstract

During an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2ed. PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strains only. PCR (under high-stringency conditions) with primers for the mur-2ed gene gave the expected amplification product only with E. durans strains. A combination of murG and mur-2ed primers in a multiplex PCR assay differentiated E. hirae from E. durans in all cases. PCR using these primers appears to be a rapid alternative for identification of E. hirae and E. durans isolates. Copyright © 2006, American Society for Microbiology. All Rights Reserved.