Genetic typification by Pulsed Field Gel Electrophoresis (PFGE) and Randomly Amplified Polymorphic DNA (RAPD) of wild Lactobacillus plantarum and Oenococcus oeni wine strains

  1. López, I. 1
  2. Torres, C. 1
  3. Ruiz-Larrea, F. 1
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

Revista:
European Food Research and Technology

ISSN: 1438-2377

Año de publicación: 2008

Volumen: 227

Número: 2

Páginas: 547-555

Tipo: Artículo

DOI: 10.1007/S00217-007-0755-0 SCOPUS: 2-s2.0-43249090107 WoS: WOS:000255627300027 GOOGLE SCHOLAR

Otras publicaciones en: European Food Research and Technology

Proyectos relacionados

Cultivos de bacterias lácticas productoras de bacteriocinas para la elaboración y conservación de vinos (AGL-200760504/ALI)

2008/00013/001

Resumen

Genetic typification of 120 bacterial isolates of Lactobacillus plantarum and Oenococcus oeni from different Rioja musts and wines was performed by numerical analysis of pulsed-field gel electrophoresis (PFGE) patterns with endonuclease SfiI, and 46 of them were also studied by randomly amplified polymorphic DNA (RAPD)-PCR. A comparative study of both typification methods applied to L. plantarum and O. oeni oenological strains was performed. Bacterial species was determined both by biochemical identification methods and by specific PCR analysis. A wide variety of restriction digest patterns were detected by PFGE among L. plantarum strains (36 unrelated patterns and one closely related pattern, out of 48 isolates), as well as among O. oeni strains (18 unrelated patterns out of 72 isolates). PFGE was shown to be a suitable method for strain differentiation and to determine which strains are present in wine fermentations, with a discriminatory power to type L. plantarum and O. oeni strains higher than that of RAPD-PCR. © 2007 Springer-Verlag.