Application of AFLPs to the characterization of grapevine Vitis vinifera L. genetics resources. A case study with accessions from Rioja (Spain)

  1. Cervera, M.-T. 2
  2. Cabezas, J.A. 2
  3. Sancha, J.C. 1
  4. Martínez De Toda, F. 1
  5. Martínez-Zapater, J.M. 2
  1. 1 Universidad de La Rioja
    info

    Universidad de La Rioja

    Logroño, España

    ROR https://ror.org/0553yr311

  2. 2 Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
    info

    Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria

    Madrid, España

    ROR https://ror.org/011q66e29

Revista:
Theoretical and Applied Genetics

ISSN: 0040-5752

Año de publicación: 1998

Volumen: 97

Número: 1-2

Páginas: 51-59

Tipo: Artículo

DOI: 10.1007/S001220050866 SCOPUS: 2-s2.0-0031853159 WoS: WOS:000075377300007 GOOGLE SCHOLAR

Otras publicaciones en: Theoretical and Applied Genetics

Repositorio institucional: lock_openAcceso abierto Editor

Resumen

AFLPs were used to characterize 67 different grapevine accessions from a collection of D.O.Ca. Rioja in Spain. A correct selection of primers and selective nucleotides allowed us to maximize the number of amplified fragments analyzed per reaction yielding an average of 100 per reaction, 49% of which were polymorphic. Based on the presence or absence of amplified fragments for each genotype resulting from a reaction with two primer combinations, we have established the genetic similarity between the different accessions in the collection. These results allowed us to resolve different genotypes maintained under the same name (homonyms) and to identify the same genotype under different names (synonyms) thus permitting the elimination of redundant germplasm. Furthermore, by providing information on more than 50 polymorphic loci per reaction, a few reactions were sufficient to identify distinct AFLP patterns characteristic of specific clones, with different agronomic and organoleptic features, belonging to the same cultivar. The possibility for clonal identification, shown here for grapevines, can have important implications in the protection and management of clonal selections.